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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۲۹-۲۹
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عنوان انگلیسی Ps-22: Synergistic Inhibitory Effects of Tamoxifen and Tranilast on VEGF Expression and Secretion in Cultured Human Breast Cancer Cells
چکیده انگلیسی مقاله Objective: Worldwide, breast cancer is a major cancer leading in both incidence and mortality in women. Angiogenesis plays essential role in breast cancer growth and progression, also a major requirement for breast tumors to successfully spread. VEGF is a main player in the neoangiogenesis of neoplastic breast tissues. Tamoxifen is a synthetic non-steroidal anti-estrogenic drug widely used for the treatment of breast cancer. Tranilast is an antiallergic agent used mainly to treat allergic diseases. The aim of present study was to examine the effects of anticancer tamoxifen and anti-allergic tranilast as a single or in combination on the proliferation and VEGF level in breast cancer cells in vitro. Materials and Methods: The human breast cancer cell line, MCF-7 was treated with graduated concentrations of tamoxifen and/or tranilast. Cytotoxic effects of tamoxifen and tranilast alone or in combination on percent cellsurvival, mRNA expression and protein levels was evaluated using lactate dehydrogenase (LDH) leakage assay, real-time RT-PCR and ELISA assay, respectively. Results: LDH assay showed that the combinational treatment of tamoxifen and tranilast showed a significant decrease in cell viability compared with tamoxifen or tranilast treatment alone, as well treatment with the tamoxifen (2 μM) and tranilast (200 μM) either alone or in combination resulted in decreased VEGF mRNA and protein expression levels significantly (indicated by realtime RT-PCR and ELISA assay). Conclusion: Tranilast could be a candidate drug for combination therapy of resistant breast cancer patients and this combination treatment can able to inhibit growth and angiogenesis of breast cancer.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/827
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