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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۰-۳۰
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عنوان انگلیسی Ps-23: Embryonic Stem Cell in Drug Toxicity: Genetically Engineered Versus Non-Genetically Engineered Cells in Cardiotoxicity Model
چکیده انگلیسی مقاله Objective: Embryonic stem cells (ESCs) have various uses in drug toxicity, as they can be easily differentiated in vitro. However, one of the major obstacles in the assessment of these differentiated cells is the presence of a heterogeneous cell population. Materials and Methods: To circumvent this problem, purified ESC-derived desired cells by means of the tissuespecific GFP and/or antibiotic resistance gene expression has been proposed. Therefore, this study aimed to assess the role of doxorubicin (DOX) in cardiotoxicity by using genetically and non-genetically engineered ESC-derived cardiomyocytes. Results: The results of this study revealed that both cell lines were suitable for evaluation of DOX cardiotoxicity. This study showed that DOX cardiotoxicity was reduced as detected by beating cardiomyocytes and caspase activity only by pretreatment with dexamethasone (DEX) and enhances cardiomyocyte-specific gene expression. Conclusion: Therefore, for the general assessment of cytotoxicity, non-genetically engineered ESC-derived cardiomyocytes are sufficient but for the molecular assessment of DOX-induced toxicity, genetically engineered purified ESC-derived cardiomyocytes are necessary.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/828
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