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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۱-۳۱
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عنوان انگلیسی Ps-27: Pancreas Extract Induces Differentiation of P19 Embryonic Stem Cells to Insulin-Producing Cells
چکیده انگلیسی مقاله Objective: So far many research groups are exploring cells to replace destroyed insulin-producing cells in the diabetic patients. One attractive approach is the generation of functional β cells from stem cells. Embryonal carcinoma (EC) stem cells are developmentaly pluripotent cells, can differentiate into all cell types under the appropriate conditions. The present study was carried out to examine the effects of mouse pancreas extract (MPE) on production of IPCs from undifferentiated P19 EC cells, using a simple nonselective inductive culture system rather than spontaneous differentiation. Materials and Methods: The cells were grown using low-attachment dishes to induce embryoid body (EB) formation. The resulted EBs cultured in a medium containing 3% fetal bovine serum, supplemented by concentration of 50, 100, 200 and 300 μg/mL MPE. Results: The results showed that P19 cells could differentiate into IPCs and form dithizone-positive cell clusters resemble those of pancreatic islets. P19-derived IPCs were immunoreactive to proinsulin+insulin and insulin receptor beta. Furthermore, expression of the genes related to pancreatic β cell development and function such as, pancreatic and duodenal homeobox 1 (pdx-1), insulin 1 (Ins1) and insulin 2 (Ins2) was observed. When stimulated with glucose, these cells synthesized and secreted insulin in a glucose-regulated manner.Conclusion: The data presented in this study showed that it is possible to generate IPCs from undifferentiated EC cells with the characteristics of pancreatic β cells. The derivation of pancreatic cells from EC cells which are ES cell siblings would provide a valuable experimental tool to study pancreatic development and function.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/832
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