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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۲-۳۲
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عنوان انگلیسی Ps-29: Similar Pattern of Epigenetic Bivalent Marks through Reprogramming of Two Human iPS Lines with Different Origins
چکیده انگلیسی مقاله Objective: Epigenetic affects many biological functions of cells like Pluripotency, differentiation and reprogramming; through molecular mechanisms such as post translational modifications (PTMs) of chromatin. Some PTMs like bivalent marks have important roles in pluripotency maintenance of embryonic stem cells (ESCs) as well as induced pluripotent stem cells (iPSCs). Bivalent marks refer to regions of epigenome with the two concurrent modifications of trimethylated lysine number 4 on histone H3 (H3K4me3) and trimethylated lysine number 27 on histone H3 (H3K27me3). These marks are usually present on regulatory regions of silent developmental genes which should be activated after onset of differentiation. Materials and Methods: Total levels of bivalent marks of H3K4me3 and H3K27me3 were quantitatively compared using chromatin-ELISA technique, in two iPS lines originated from a differentiated ESC and a fibroblast cell line, respectively. Results: Results clearly showed similarity in pattern of bivalent marks through reprogramming of both differentiated ESC and committed fibroblast cells. While the transcription activating mark of H3K4me3 increased significantly through reprogramming of cells the repressing mark of H3K27me3 decreased during the process. Conclusion: Altogether, it can be concluded that bivalent marks have important role in epigenetic regulation of reprogramming in induced pluripotent stem cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/834
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