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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۳-۳۳
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عنوان انگلیسی Ps-30: Non-smad TGF-Beta Signaling Pathway Govern Adult Stem Cell State with The Cooperation of SLUG and SOX9
چکیده انگلیسی مقاله Objective: Induction of adult stem cell state may open a novel perspective in medicine. Autoregulatory networks involved in determination and maintenance of embryonic stem cell (ESC) state have been investigated. These networks that can be induced by a set of master transcription factors are the source of induced pluripotent stem cells (iPSCs) induction and maintenance. iPSCs are novel powerful tools in regenerative medicine, while their usage overcomes the immunogenicity and ethical problems of ESCs. Although master transcription factors should be present in adult stem cells, our knowledge of such network is poor. Transforming growth factor (TGF)- beta superfamily has shown to be crucial in development, homeostasis and malignancies. In malignancies, TGF-β promotes metastasis by the induction of epithelial to mesenchymal transition (EMT), a process involved in gastrulation, wound healing and induction of cancer stem cells. Interestingly, EMT has been connected to stem cell traits of epithelial tissues by induction of the stemness. Materials and Methods: By overexpression and knockdown of SLUG and SOX9 TFs, we measure TNC and POSTN expression by real-time PCR and Western blot analysis. SLUG and SOX9 colocalization and co-immunoprecipitation is going to be tested. Stemness analysis in mice is the in vivo confirmation of the regulatory effects of SLUG and SOX9, by fat-pad transplantation of treated cells. Results: The proposed novel non-smad TGF-beta signaling connects intracellular signaling cascade to the regulation of extracellular matrix proteins involved in stem cell niche, and shows an emerging autoregulatory network in adult stem cells. Conclusion: Here, a proposed novel non-smad TGF-beta signaling connects intracellular signaling cascade to the regulation of extracellular matrix proteins involved in stem cell niche, and shows an emerging autoregulatory network in adult stem cells. Via this view point, induction of adult stem cells can be a novel area of research with several capacities in regenerative medicine. Regeneration of damaged skin, resected bowel and excised breast can be among achievements of this finding. Key regulatory networks involved that controls stemness in adult stem cells need to be discovered. Master transcription factors that regulate these networks may be the central mystery to be solved.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/835
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