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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۴-۳۴
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عنوان انگلیسی Ps-33: Effect of Pentoxifylline in The Presence of Staurosporine on Neuronal Differentiation in Mouse Bone Marrow Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: The in vitro generation of neural-like cells from stem cells is a promising approach to produce cells suitable for neural tissue repair and cell-based replacement therapies in neurological damages. Available methods to promote stem cells differentiation towards neural lineages attempt using the various compounds had been partly successful. This study was undertaken to evaluate the effects of different concentrations of pentoxifylline in the presence of staurosporine on neuronal differentiation in mouse bone marrow mesenchymal stem cells. Materials and Methods: In the present study, mouse mesenchymal stem cells are isolated from an aspirate of bone marrow harvested from the tibia and femoral marrow compartment. Cells were cultured in DMEM culture medium supplemented with 10٪ FBS for 72 hours. After the first passage, in experiment group cells were pretreated with different concentrations of pentoxifylline (10-6, 10-5, 10-4M, treatments I, II, III; respectively), Then cells were treated in the same concentrations of pentoxifylline in the presence of 100nM staurosporine. Non-pretreated cells with pentoxifylline were considered as control group. Neuroglial differentiation was performed by total neurite length measurement GFAP and β-tubuline immunocytochemistry staining. Results: After 12 hours Total neurite length measurements after 6 hr in control group, treatments I, II and III was 286.839, 309.166, 330.49, 334.509 μm; respectively. Pentoxifylline at either of low or high concentration resulted in increase of neurite elongation (p< 005). Pentoxifylline at either of low or high concentration enhanced Tubulin Beta III and GFAP protein expression compared with control (p< 005). Immunocytochemistry data showed that pentoxifylline in treatment II increased Tubulin Beta III and GFAP protein expression compared with other treatments in experiment group. Conclusion: It suggests that pentoxifylline stimulate the neurial differentiation by enhancing neurite elongation and neuronal protein expression in mouse bone marrow mesenchymal stem cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/838
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