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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۵-۳۵
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عنوان انگلیسی Ps-34: Effect of Pentoxifylline in The Presence of Staurosporine on Mouse Bone Marrow Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Bone marrow mesenchymal stem cells can be differentiated into neuronal and glial cell types under appropriate experimental conditions. Pentoxifylline induce its effects by several mechanisms including translocation of extracellular calcium, increasing in cAMP caused by inhibition of phosphodiesterases. This study was undertaken to evaluate the effects of different concentrations of pentoxifylline in the presence of staurosporine on mouse bone marrow mesenchymal stem cells (mBMSCs). Materials and Methods: In the present study, mouse mesenchymal stem cells are isolated from an aspirate of bone marrow harvested from the tibia and femoral marrow compartment. Cells were cultured in DMEM culture medium supplemented with 10٪ FBS for 72 hours. After the first passage, in experiment group cells were pretreated with different concentrations of pentoxifylline (10-6, 10-5, 10-4M, treatments I, II, III; respectively), Then cells were treated in the same concentrations of pentoxifylline in the presence of 100 nM staurosporine. Non-pretreated cells with pentoxifylline were considered as control group. The viability of cells was assessed by neutral red uptake assay and cell death was performed by PI/Hoechst staining assay. Results: The viability of cells in control group was higher than experiment group in all treatments and the lowest viability was in experiment group, treatment III (81.75%), (p< 0.05). The highest cell death after 6, 12 and 24 hr observed in treatment III (28.61%, 33.17% and 41.98%; respectively) and the lowest cell death observed in control group 917.12%, 17.35% and 18.9%; respectively) (p< 0.05). Conclusion: According to our results, pentoxifylline increased cell death and in mBMSCs in a time and dose dependent manner.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/839
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