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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۵-۳۵
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عنوان انگلیسی Ps-36: Higher Expression of Signal Transducers and Activators of Transcription-3 (STAT-3) in Gastric Cancer Stem Like Cells (GCSCs)
چکیده انگلیسی مقاله Objective: STAT-3 transcription factor participates in some biological phenomenon including inflammation, anti-tumor immunosuppression, cellular proliferation and differentiation. Moreover, it helps to maintain pluripotency of embryonic stem cells and there are some recent evidences of its footprint in stemness properties of cancer initiating cells. In the present study, the expression of STAT-3 was evaluated in the spheroid bodies derived from MKN-45 cell line as cancer stem like cells and the results compared to parental cells. Materials and Methods: Spheroids obtained from non adhesive culture of cells in serum free media supplemented with EGF, bFGF (20 ng/ml) and B27 (2%). Gastro-spheres were characterized by expression of CD24, CD44, CD133, EpCAM, CD71, CD73, CD90 as putative CSCs markers; Nanog, Oct-4, Sox-2, Klf-4 and c-Myc as stemness related genes and drug resistancy to DTX. Finally, the expression of STAT-3 tested by immunofuorescence staining and real- time PCR. Parental cells were used as control group. Results: Gastro-spheres (passage 4) showed higher expression of CD44, CD24, CD71, and over expressed Nanog, Oct-4, Sox-2, Klf-4 and c-Myc genes than parental cells. Immunostaining of STAT-3 demonstrated that both spheroid cells and parental cells expressed STAT3 at protein level, however, its mRNA level in gastro-spheres was higher than parental cells. Conclusion: Spheroid formation provide an applicable method to isolate cancer stem like cells from MKN-45 cell line and the findings if support by further examinations may imply to roles of STAT-3 in GCSCs features, as recently seen in brain and glioblastoma CSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/841
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