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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۶-۳۶
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عنوان انگلیسی Ps-39: Therapeutic Application of MicroRNAs Improves Stem Cell Function: Overexpression of MiR-210 Enhances Stemness Status of Human Wharton’s Jelly Stem Cells
چکیده انگلیسی مقاله Objective: MicroRNAs (miRs) have the potential to control stem cells fate decisions through regulation of gene expression. miR-210 is one of this agents that is shown to be associated with VEGF during angiogenesis in endothelial cells and promote survival and number of vascular colony forming units of haematopoietic stem cells (HSCs). Accordingly, we hypothesized that over expression of miR-210 may also promote human Wharton’s jelly stem cells (hWJSCs) proliferation, while inhibit the cells senescence and apoptosis through targeting the genes involved in these processes. Materials and Methods: We evaluate the direct effect of over-expression of miR-210 in expansion potential and on the proliferation rate of hWJSCs. On day 5 of expansion, cells were seeded in media and transfected with miRs at a final concentration of 160 nM using Lipofectamine. To silence miR-210 expression, cells were treated with specific Anti-miR Inhibitor, hsamiR- 210. Results: After 48 hours incubation, hWJSCs exhibited effectively expanded potential (15- to 20-fold) in culture medium containing miR-210, while miR-210 inhibitor abrogated significantly the proliferator capacity of treated cells. Conclusion: Collectively, these observations highlight a critical role for microRNAs in enhancing the proliferation property of expanded cells, and identify miR-210 as a potential molecule and a therapeutic target to enhance hWJSCs function in treatment of degenerative disease.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/844
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