این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۸-۳۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-42: The Effect of Retinoic Acid in Differentiation of Male Mouse Adipose Tissue Derived-Mesenchymal Stem Cells into Germ Cells
چکیده انگلیسی مقاله Objective: Recent publications regarding to differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. In vitro production of germ cells not only provides new approaches to infertility, but also improves understanding differentiation process of male and female germ cells. Because using embryonic stem cells for this purpose has been associated with tumorogenesis and also ethical criticisms, the mentioned cells were suggested to be replaced with some adult multipotent stem cells.To find appropriate non invasive source replacement for embryonic stem cells in this study we designed to evaluate differential potentials of adipocyte derived stem cells (ADMSCs) to germ like cells. Materials and Methods: To find differentiation capability, after providing purified ADMSCs differentiation to osteoblast and adipocyte was confirmed by using appropriate culture medium. Superficial markers for mesenchymal stem cells (expression of CD90 and CD73 and nonexpression of CD45 and VEGFR2) were investigated by flowcytometry to confirm mesenchymal lineage production. The cells were differentiated to germ cells in mediums containing Retinoic Acid for 7 days. To evaluate germ cells characteristic markers (Mvh, Dazl, Stra8, Scp3) flowcytometry, imunoflorescence and RT-PCR were used. Results: Presentation of stem cell superficial markers (CD90, CD73) and absence of endothelial and blood cell markers (VEGFR2, CD45) were confirmative for mesenchymal origination of these cells. The cells were able to differentiate into osteoblast and adipocyte cells. This fact was representative for multipotential entity of the examined cells. The flowcytometry, imunoflorescence and RTPCR results showed remarkable expression of germ cells characteristic markers (Mvh, Dazl, Stra8, Scp3). Conclusion: By this study, it was found that germ cell markers were expressed in ADMSCs aftter adding exogenous Retinoic Acid into culture medium.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/847
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات