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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۸-۳۸
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عنوان انگلیسی Ps-44: Neural Stem Cell Culture in Alginate Scaffold fold
چکیده انگلیسی مقاله Objective: The neurosphere assay is usually used to culture neural stem cells (NSCs). In this system, cells are under the least physical interaction with their environment. Using a more solid matrix might affect cellular behavior in terms of proliferation and differentiation that could benefit selection of cells with different properties. Alginate is a polysaccharide that is used in 3D cultures.Here, we evaluated NSCs expansion in different alginate concentrations (0.25, 0.5, and 1 %). Materials and Methods: First, NSCs from the E-14 mouse brain were cultured in different Alginate concentrations (0.25, 0.5 and 1%) and a control group. After 5-6 days, the cultures were evaluated for neurosphere growth and the sphere forming frequency was determined for each condition. Results: Evaluating the cultures from different groups showed that neurospheres could grow in all conditions but the sphere forming frequency was the highest in control comparing to the Alginate groups. In Alginate groups, it was revealed the higher the Alginate concentration, the lower the sphere forming frequency. Furthermore, all spheres from both control and Alginate groups could be serially passaged. Comparing the size of spheres, it was evident that the spheres in higher concentration of Alginate were larger than the control. Conclusion: Our study shows that the mechanical effects of alginate 3D scaffold reduce the sphere forming frequency of neural stem cells. At the same time growing NSCs in Alginate scaffold results in larger spheres that could be due to selection of bona fide NSCs in Alginate culture comparing to the control.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/849
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