این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۳۹-۳۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-46: Study of The Expression of A Pluripotency-Long Non Coding RNA in Embryonic Germ Cells
چکیده انگلیسی مقاله Objective: Although a wealth of information currently exists for genetic networks involved in pluripotency of mouse embryonic stem cells mESCs, less is known about mouse embryonic germ cells (mEGCs). Especially To date more studies have focused almost on protein-coding transcripts. However, recent transcriptome analyses show that the mammalian genome contains thousands of long noncoding RNAs (LncRNAs), many of which appear to be expressed in a developmentally regulated manner. The purpose of this study is to identify other key regulators of pluripotency in mEGCs. Materials and Methods: P19 cells cultured in α-MEM supplemented with 10% heat-inactivated fetal bovin serum. RNA extracted from P19 cells in exponential phase. Quality and quantity of RNA has examined by gel electrophoresis and Nano drop. To study expression of LincRNA1283(large intergenic non coding RNA) at RNA level, at first we found the sequences of this LincRNA in papars and designed specific primer by Primer premier software. Specific cDNA was synthesized by revers primer and hemi nested PCR was done. To confirm amplified PCR product, it was digested and also sequenced. Results: Hemi nested PCR for lincRNA 1283 produced a fragment of 285 bp size. Its sequence was also confirmed by sequencing. Conclusion: As a result in this study, we showed expression of lincRNA 1283 at mRNA level in P19 cell as pluripotent cell.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/851
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات