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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۰-۴۰
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عنوان انگلیسی Ps-140: The Effect of Storage Temperature on Cord Blood Hematology Parameters Over Time
چکیده انگلیسی مقاله Objective: Standardized procedures for cord blood (CB) storage before and during cryopreservation as well as thawing are still controversial. This work is concerned with the effect of CB storage temperature and storage duration through the stages before cryopreservation on cell recovery, viability, aggregation of cells and hematopoietic reconstitution potential. Materials and Methods: Total of 30 CB samples were stored at 4°C, 12°C, 20°C, and 37°C for different periods of time after collection and before cryopreservation. Subsequently, these samples were compared in terms of recovery, viability, aggregation, and hematopoietic reconstitution potential. Results: Duration and temperature used for CB storage affect cell recovery, viability, and aggregation of cells. Results were remarkably stable at 12-20 C° for 48-72h. Conclusion: CB cells demonstrate different degrees of vulnerability to in-vitro cell death depending on the duration and temperature applied at the time of storage and before cryopreservation. Our results suggest that the best temperature before cryopreservation is 12- 20°C up to 48-72h.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/942
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