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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۲-۴۲
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عنوان انگلیسی Ps-51: The Effects of Extremely Low-Frequency Pulsed Electromagnetic Fields on Osteogenic Differentiation
چکیده انگلیسی مقاله Objective: Osseous tissue Repair and regeneration is a big challenge in orthopedic and maxillofacial surgery. Surgical interferences are usually needed in most abnormalities like trauma and infections and Bone grafts are usually used as a treatment for such diseases. Tissue engineering makes it possible to cure different tissue abnormalities with no need to surgery by using the autologous grafts which are the stem cells of the patient that undergone some types of stimulation. Recently, Applying electromagnetic fields enter the usage of physical signals in the field of tissue engineering. Transmission of physical signals is related to change in structural organelles and proteins will translate to biochemical signals. Extremely low magnetic field (ELF) with low frequencies can mediate biologic responses. Electromagnetic waves can increase intra cellular calcium and cause activation of calmodulin and increase in expression of osteogenic marker genes. So magnetic fields could play decisive role in promotion of differentiation pathways. Materials and Methods: Four weeks old mature rats were anesthetized and bone marrow stem cells were isolated and cultured in α-MEM medium containing 15% FBS. Flow-cytometry (CD45 and CD90) and multipotential tests confirmed the identity of the isolated cells. Pulsed electromagnetic field (PEMF) was applied with 0.2mT intensity and 15Hz frequency; 6 hours per day. The test groups were included of chemical, magnetic and magnetic/chemical. In magnetic/chemical group, osteogenic induction medium (containing 1 μM dexamethasone, 10 mM β-glycerophosphate and 0.1 mM ascorbic acid) were used with magnetic exposure. Gene expression in magnetic- chemical group was done in day 7 and 10 after addition of osteogenic medium. Expression of osteoblastic marker genes (OCN and Runx2) was evaluated by TaqMan Real-Time PCR. Alizarin Red S staining showed the formed calcium nodules. Results: Real-time results showed the expression levels of Runx2 and OCN were much more increased in magnetic/ chemical group in comparison to magnetic and also chemical ones alone. Conclusion: Combination of electromagnetic field and chemical factors is much more suitable for directing osteogenesis and these two elements enhance each other.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/855
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