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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۳-۴۳
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عنوان انگلیسی Ps-53: Investigations on Amount of Secreted Nitric Oxide from Endothelial Cells in Response to Shear Stress
چکیده انگلیسی مقاله Objective: Nitric oxide (NO) as an intracellular messenger from the vascular endothelial cells (ECs) plays crucial roles in regulation of regional blood flow by vasodilatation and inhibition of platelet aggregation and adhesion leading to prevention of atherosclerosis and thrombosis. ECs are constantly exposed to blood flow shear stress, which regulate important physiological blood vessel responses such as NO release. The present study aims to address the effects of shear stress on changes in amount of NO secretion from ECs. Materials and Methods: Human Umbilical Vein Endothelial Cells (HUVEC, NCBI Code: C554) were obtained from National Cell Bank of Iran and cultivated in DMEM (Gibco, NY, USA), supplemented with 10% FBS (Gibco, NY, USA) and penicillin/streptomycin (Gibco, NY, USA) at 37°C and 5% CO2 incubator. Silicone tube with 2mm-ID (dedicated from Research Center for New Technologies in Life Science Engineering) were used as scaffold. HUVECs were cultured on the scaffold surface modified by collagen type I solution coating. By peristaltic pump and a designed perfusion bioreactor, we applied 1.8 dyne/cm2 shear stress to HUVECs and NO concentration were measured at different times in an hour by Griess reagent and read with Elisa reader Results: HUVECs released basal level of NO at static (control) condition. At all times NO amount was more than control condition at the same time. Applying 1.8 dyne/cm2 shear stress caused a rapid 7-fold increase in NO response of the cells at first 5 minute in an hour compared with control group (p< 0.05). Conclusion: The results showed that HUVECs respond to flow shear stress by release of more NO compared with static condition. Results of this study suggest that applying the shear stress to tissue engineered blood vessels can decrease the possibility of thrombosis through increasing the NO release.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/857
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