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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۵-۴۵
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عنوان انگلیسی Ps-57: Expression of C3(ETF3) in Co-Culture of Ovis Aries Embryos with Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Co-culture reduces the fragmentation rate of human embryos are cultured in vitro and increases the blastulation rate. The oviduct-derived embryotrophic factors (ETFs) affected the development of embryos. ETF- 3 is most abundant among the three ETFs. This factor is a mixture containing complement protein 3 (C3), and its derivatives, C3b and iC3b. Mesenchymal stem cells (MSCs) could improve meiotic maturation in vitro and subsequent embryo developmental potential by secretion of various growth factors and several components. In this study with this assumption that co-culture enhances the vitality of the embryo at blastocyst stage, we use MSCs in culture medium of embryo and detect expression of C3 (ETF3). Materials and Methods: Five groups’ cells in three clusters (mesenchymal stem cell, ovis embryo in 8- to 16-cell and co-culture of Mesenchymal stem cell and embryo cells) were analyzed. Total RNA was extracted using Trizol reagent according modified manual instructed in kit. RT-PCR was used to confirm the expression of C3 (ETF3). All amplified fragments were analyzed by electrophoresis on a 6% polyacrylamide gel and sequenced to verify PCR-products. Results: The data of this study was very significant. Although there was expression in three groups of cells and results were demonstrated via Sequencing, but it must be mentioned, we could use stem cell as a source of production. On the other hand, if the embryo could not express this factor, using of these cells was helpful. Also the other possibility is that the post-translational mechanisms and intercellular interaction regulate using of ETF3 by embryo. Conclusion: Co-culture of embryo with MSc can improve vitality of embryo in a gene expression manner. The detailed influence of MSCs on blastulation rate and subsequent embryo developmental await further investigation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/861
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