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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۶-۴۶
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عنوان انگلیسی Ps-64: Differentiation of Mesenchymal Stem Cells to Smooth Muscle Cells on Nanostructured Scaffold for Bladder Tissue Engineering
چکیده انگلیسی مقاله Objective: Severe bladder dysfunction can be induced by disease or surgical intervention altering the normal pattern of storage and voiding. Severe cases of bladder failure typically do not respond to the most conservative treatment. Currently, the treatment of choice in these patients is an enterocystoplasty, a surgical enlargement of the bladder using intestinal tissue. The primary aim of the surgical reconstruction is to increase bladder capacity and compliance. This method fails to restore emptying function. Furthermore, enterocystoplasty is associated with numerous complications such as metabolic disturbance, increased mucus production, infections and even malignant diseases. Tissue engineering, using autologous cells for implantation might offer a solution to this problem. Recently, several studies have confirmed feasibility of bladder reconstruction using engineered segments which were formed using bioMaterialss seeded with autologous cells in vitro. Mesenchymal stem cells (MSCs) have potential to differentiate into all three germ layers and compared with embryonic stem cells demonstrate considerable advantages, including stable differentiation into specific cell lineages, no transdifferentiation into a malignant phenotype, no requirement for the sacrifice of human embryos for their isolation and no or little immune rejection. Materials and Methods: In this study, the hybrid nanofibrous scaffold of polycaprolactone/polylactic acid was synthesized by electrospining method. MSCs were seeded on the prepared nanostructured scaffold.Transforming growth factor beta1(Tgf-β1) and ascorbic acid were added to the culture medium in order to induce differentiation of MSCs to smooth muscle cells (SMCs). Results: Adhesion and proliferation of the cells were successfully demonstrated and finally the potential of the differentiation of MSCs to SMCs were investigated. Conclusion: Our results demonstrated that the cultured MSCs on the nanostructured scaffold possess the capability of differentiation towards SMCs, showing the potential use of the prepared scaffold in bladder tissue engineering.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/868
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