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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۶-۴۶
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عنوان انگلیسی Ps-65: Expansion of Mesenchymal Stem Cells on Porous Chitosan-Gelatin Microcarrier in Suspension Culture
چکیده انگلیسی مقاله Objective: Mesenchymal stem cells (MSCs) have potential clinical utility in the treatment of a multitude of ailments and diseases, due to their relative ease of isolation from patients and their capacity to form many cell types. However, MSCs are sparse, and can only be isolated in very small quantities, thereby hindering the development of clinical therapies. Furthermore, because of the specific properties of these cell populations, there is a need to investigate novel cell culture strategies adapted from established dynamic cultivation practices. The use of microcarrier-based stirred suspension cultures to expand stem cell populations offers an appropriate approach to overcome this problem. Materials and Methods: In the current study porous chitosan-gelatin microcarriers were successfully fabricated for use in cultivation of stem cells. A modified syringe pump method followed by freeze-drying was used for fabricating the porous chitosan-gelatin microcarrier beads. Various factors that influenced the preparation of microcarriers were studied and the conditions were optimized to prepare microcarriers having the desired size, porosity, mechanical elastisity and biocompatibility. MSCs were loaded on the microcarriers with standard culture protocol commonly reported in the literature in a 48-well plate and then the plate put onto a mini-rocker to facilitate expansion of MSCs. Results: The behavior (attachment, expansion and morphology) of the cells on the microcarriers were investigated in order to check the suitability of the prepared microcarriers for stem cell cultivation. The viability of immobilized cells on the microcarriers was investigated by an MTT assay. Conclusion: The observation results showed that cellular morphology was homogenous around the microcarriers and MSCs expanded gradually on the microcarriers surface.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/869
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