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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۷-۴۷
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عنوان انگلیسی Ps-66: Comparison of The Chromosomal Stability of Mouse Embryonic Stem Cell in Medium Containing R2i (TGF-ß and ERK1,2 inhibitors) with Medium Containing 2i (GSK-3 and ERK1,2 inhibitors)
چکیده انگلیسی مقاله Objective: Mouse embryonic stem cells are pluripotent cells which are derived from the blastocysts of the preimplantation embryos. The success rate in generating embryonic stem cells in some mouse stains like BALB/c, C57BL/6, DBA/2 has been very low and altered by the genetic variations. Nowadays the researchers have succeeded in producing embryonic stems cells from these resistant strains by identifying the cell signaling pathways involved in pluripotency maintenance and using differentiation inhibitory small molecules. In this study the chromosomal integrity of mouse embryonic stem cells after exposure to two different mediums containing ERK1,2 and GSK3 pathways inhibitors (2i) and ERK1,2 and TGFβ pathways inhibitors (R2i) were investigated. Materials and Methods: 9 cell lines were derivate from BALB/c, C57BL/6 and DBA/2 mouse strains (3 cell lines from each strain). These lines were cultured in R2i medium and after 10 passages, were studied for chromosome stability by G-band karyotype analysis. The normal karyotype lines were selected and divided to two groups, each were cultured in 2i and R2i medium for 20 passages. Then these two groups were karyotyped to evaluate their chromosome integrity. Results: The rate of numerical and structural abnormalities in the proliferated cells in 2i medium were significantly higher than that of the cells on the R2i medium (in comparison with the primary10 passages in R2i medium). The mean of chromosomal abnormalities of all proliferated cells in 2i medium were significantly higher than the cells in R2i medium (39.33 and 22.66% respectively). It should be mentioned that the BALB/c strain showed the least chromosomal abnormality percentage, while the DBA/2 strain had the most abnormality. Conclusion: Inhibiting GSK3 pahtway during cell divisions in mouse embryonic stem cells may have more destructive effect on chromosomal integrity than inhibiting TGFβ.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/870
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