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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۴۷-۴۷
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عنوان انگلیسی Ps-67:In Vitro Fertilization Rate Improvement and Decrease of Apoptosis Incidence in Rams Following Feeding with Omega-3 Source
چکیده انگلیسی مقاله Objective: 0mega-3 is found in high levels in mammalian spermatozoa and it could improve sperm classical parameters that correlate closely with fertilization. The aim of this study was to evaluate the apoptosis incidence and in vitro fertilization (IVF) rate following ram diets supplementation with omega-3 source. Materials and Methods: Eight of 2-3 years old Zandi rams (live weight: 65 ± 10kg)was randomly assigned into two groups: control (CTR) and fish oil (FO). Energy, protein and vitamin E supplement level was similar in both groups. Rams in FO group received maintenance diet in associated with 35gr/day/ram fish oil. diet were fed for 70 days. After adaptation period (21 d), semen was collected from each ram by artificial vagina and transferred to laboratory. Fertilization capacity of swimup processed semen was only assessed at the first and last day of sampling by in vitro fertilization technique, but apoptosis incidence was evaluated in every week of sampling by flow cytometry technique. Results: Flow cytometry results showed that totally the occurrence of apoptosis in the FO group was lower than CTR group. This difference was only significant in the 3 rd week of sampling (p≤0.05). Also two cell stage embryos rate following IVF in two groups indicated significant higher rate in FO group as compared with the CTR group (p≤0.05). Conclusion: Overall, it could be concluded that adding fish oil to rams diets not only, could reduce the Probability of apoptosis incidence, but also could increase the fertilization rate. It seems that omega-3 could be an appropriate additive in ram diet
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/871
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