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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۰-۵۰
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عنوان انگلیسی Ps-71: Evaluation of miR-210 Effect on Proliferation and Survival of Mouse Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Bone marrow derived mesenchymal stem cells (MSCs) are a population of multipotent progenitors which have the capacity of proliferation and differentiation into mesenchymal lineage cells. Micro-RNAs are endogenous 22 nt RNAs that can play important roles in some processes such as proliferation and differentiation. We hypothesized that miR-210 could help for better prolifratation MSCs since this miRNA can activate HIF pathway. So MSCs could preserve their differentiation ability under normoxic conditions without any growth factors. Materials and Methods: MSCs isolated from C57 BL/6 mice by flushing its femurs into cell culture media. After 72 hours MSCs which are plastic adherent cells were attached to the flask and nonadherent cells were removed. Subsequently MSCs differentiated into osteocytes and adipocytes with specific differentiation media to confirm their identity and multipotency. Also we were inserted miR-210 in Lentiviruse vectors and affected them on MSCs. The expressions of miR-210 and HIF-1α in each passage were evaluated by Real time PCR. Results: Comparison between miR-210 infected MSCs and control cells showed that miR-210 has ability to increase proliferation of MSCs while maintained their ability to differentiate into adipocytes and osteocytes. The expression of miR-210 and HIF-1α were up regulated in each passage. Conclusion: In order to important roles of MSCs, proliferation and maintenance of their ability are necessary. We showed that miR-210 has ability to proliferate MSCs without any effect on their differentiation abilities. Morther studies are needed for evaluation of probably miR-210 effects mechanism on MSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/875
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