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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۰-۵۰
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عنوان انگلیسی Ps-72: Culture and Differentiation of Mouse Neural Stem and Progenitor Cells in A Polymeric Nano-Poly-L Lactic Acid
چکیده انگلیسی مقاله Objective: The selection of a good quality scaffold is an essential strategy for tissue engineering. Ideally, the scaffold should be a functional and structural biomimetic of the native extracellular matrix and support multiple tissue morphogenesis. Neural stem/progenitor cells (NSCs) are intrinsically capable of differentiating into different neural cell types within the nervous system, offering prospects for NSC-based cell therapies to treat neurodegenerative diseases. In this study, an attempt was made to develop porous polymeric Nano-fiber scaffold using a biodegradable Poly (l-lactic acid) (PLLA) for in vitro culture of neural stem and progenitor cells. Materials and Methods: To fabricate Nano-fiber, we used electrospinning techniques. The physico-chemical properties of the scaffold were fully characterized by using scanning electron microscopy. NSCs were isolated from the subventricular zone of the adult mouse brain and cultured in the scaffold and then the viability, proliferation and differentiation of these cells determined via both of Immunostaining and MTT assay. Scanning Electron Microscopy (SEM) was also used to observe the morphology of the cells cultured on PLLA fibrous scaffolds. Results: DAPI and Immunostaining revealed the neuronal differentiation and adhering of cells on the surface of the electrospun scaffolds. Blue stained cells were found in Nano-fiber indicating that the scaffold had provided basic cell attachment. The MTT assay results showed that the absorption in 570 nm increased during 2 to 5 days. The morphology of differentiated cells was evaluated by SEM. Conclusion: Nano-Scaffold PLLA due to the biocompatible properties is an appropriate structure for proliferation, differentiation and normal growth of the neural stem and progenitor cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/876
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