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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۱-۵۱
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عنوان انگلیسی Ps-73: Inside into ID Basic Helix-Loop-Helix Genes Expression during Retinoic Acid Induced Differentiation of Human Embryonic Carcinoma Cells
چکیده انگلیسی مقاله Objective: The basic helix-loop-helix (bHLH) family of transcription factors are thought to affect the balance between cell proliferation and differentiation in coordinated regulation of gene expression and cell lineage commitment in most mammalian tissues. Helix-loop-helix ID (Inhibitor of DNA binding) proteins are distinct from bHLH transcription factors in that they do not possess basic domain necessary for DNA binding and function as dominant negative regulators of bHLH transcription factors . It is supposed that various members of the ID family have active roles in embryonic stem cell self-renewal and also in a range of human tumors. Since embryonal carcinoma (EC) cells are malignant but their terminally differentiated derivatives are not, understanding the expression profile of these embryonal cells may be of value for therapeutic purposes in embryology and development. These cells can be differentiate into neural linage upon exposure to retinoic acid (RA), an analogue of vitamin A, which is a robust inducer during normal mammalian development. Materials and Methods: In the current work, differentiation was induced by retinoic acid in a human embryonal carcinoma cell line, named NT2/NTERA2. The cells were harvested at 0, 1, and 3 days of RA induction. The mRNA expression levels of all four ID genes were quantitatively evaluated before and after RA treatment by real time-PCR technique (qRT-PCR). Results: The results declared significant expression levels of ID gene family in embryonal carcinoma cells. During development although onset of differentiation showed increase in the levels of ID genes, the levels of their mRNA decreased by day 3 of differentiation. Conclusion: Elucidation of the detailed pattern of ID genes expression would reveal novel roles for promoting our understanding of how differentiation and proliferation are regulated.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/877
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