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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۱-۵۱
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عنوان انگلیسی Ps-74: Intra-Renal Arterial Injection of Autologous Bone Marrow-Mesenchymal Stem Cells Ameliorates Cisplatin-Induced Acute Kidney Injury in A Rhesus Macaque Mulatta Monkey Model
چکیده انگلیسی مقاله Objective: Acute kidney injury (AKI) is a potentially devastating disease in clinical medicine. However, no specific therapy improves the rate or effectiveness of the repair process after AKI. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been proven to be benefit to the renal repair after AKI in different experimental models of rodent models, but the consequence of these results to large animals and eventually, to humans remains unknown. Thus, the aim of this study was to assessment the effect of autologous rhesus Macaque monkey BM-MSCs transplantation in cisplatin-induced AKI. Materials and Methods: BM-MSCs were characterized for their growth characteristics, differentiation potential, immounophenotypic properties and chromosome content. According to design procedure, monkeys were divided into control, vehicle and cell transplantation (Cell Tx) groups and exposed to cisplatin 5mg/kg as intravenous for induce of AKI. Control animals were not treated with anything but cell Tx and vehicle animals were treated with intra-renal arterial injection of autologous BM-MSCs and normal saline, respectively. For cell tracking with magnetic resonance imaging (MRI), BM-MSCs labelled with nanoparticles superparamagnetic iron oxide (SPIO). Results: Labelled BM-MSCs were found in both glomeruli and tubules. Transplantation of 5×106 cell/kg ameliorated renal function during first week as reflected by significantly lower serum creatinine and urea values and higher urine creatinine and urea clearance without hyponatremia, hyperkalemia, proteinuria and polyuria to 3 months in compare with vehicle and control groups. BM-MSCs markedly accelerated present of Foxp3+ regulatory T (Treg) cells in response to cisplatin-induced damage, as revealed by higher numbers of Foxp3-positive cells within the tubuli with respect to cisplatintreated monkeys in control and vehicle groups. However, BM-MSCs did not repair kidney parenchyma and as partially to modulate ultrastructure of kidneys. Conclusion: These data demonstrate that BM-MSCs in this unique cisplatin-induced AKI large-animal model, did exhibit reparative and protective properties. Keywords: Rhesus Macaques’ Bone Marrow Mesenchymal Stems Cells (rmBM-MSCs), Acute Kidney Injury (AKI), Flowcytometry, Histopathology, Immunohistochemistry, Transition Electron Microscopy (TEM), Superparamagnetic Iron Oxide (SPIO), Magnetic Resonance Imaging (MRI)
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/878
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