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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۱-۵۱
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عنوان انگلیسی Ps-76: The Effect of Cerebrospinal Fluid on Neural Differentiation of Human Umbilical Cord Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Wharton’s jelly is the gelatinous connective tissue from umbilical cord and is composed of umbilical cord mesenchymal stem cells, collagen fibers, and proteoglycans. The stem cells in WJ of the umbilical cord have properties that make them of interest. For example, they are simple to harvest through noninvasive methods, provide large numbers of cells without risk to the donor, the stem cell population may be expanded in vitro, cryogenically stored, thawed, genetically manipulated and differentiated in vitro. Materials and Methods: The cells in passage 2 were induced into neural differentiation with different concentration of human Cerebrospinal fluid. Differentiation along the neural lineage was documented by expression of three neural marker Nestin, MAP2 and GFAP for 21 days. The expression of the identified genes was confirmed by Reverse Transcriptase PCR. Results: RT-PCR showed that Cerebrospinal fluid could promote expression of Nestin, MAP2 and GFAP mRNA in an dose-dependant manner, especially at the concentration of 200 μl/ml. Conclusion: CSF induce neurogenesis of human Wharton’s jelly stem cells, which encourage tissue engineering applications of these cells in neurodegenerative defects and traumatic brain injury.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/880
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