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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۱-۵۱
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عنوان انگلیسی Ps-77: Mesenchymal Stem Cell Derived Microvesicles:Trophic Shuttles for Enhancement of Sperm Quality
چکیده انگلیسی مقاله Objective: Diminishing sperm quality during cryopreservation process is the major problem in the field of reproductive biotechnology. The final consequence of such destructive events would be a complete or partial loss of sperm’s fertility. Therefore, rehabilitation of such affected sperms would be a crucial aim to improve sperms quality and fertility. A variety of evidence has indicated that secreted factors from mesenchymal stem cells (MSCs) are involved in regeneration of injured endogenous cells. Recently, MSCs-derived microvesicles (MVs) have been known as potent carriers of such trophic molecules. So, based on the demonstrated roles of these trophic molecules in improvement of sperm’s motility, capacitation and survival, here, we introduce mesenchymal stem cell-derived microvesicles as potent trophic organelles to enhance sperm quality and fertility potential. Materials and Methods: Sperms were obtained from cauda epididymis of 18 weeks old male Wistar rats. Collected sperms from each rat were equally assigned to four separate groups. Following suspension in cryoprotectant extender, sperm were untreated or treated with increasing concentrations of MSC-derived MVs (25, 50 and 100μg). After 3 hours incubation, sperms were cryopreserved. The frozen-thawed sperms were undergone to assess for viability, motility and anti-oxidant capacity parameters. Consequently, the frequency changes of apoptotic and necrotic sperms and expression levels of sperm surface adhesion molecules (CD29, CD44, ICAM-I and VCAMI) were assessed by flow cytometery. Results: Results showed the enhanced quality and adhesive properties of cryopreserved sperms following treatment with MSC-derived MVs. Conclusion: Based on the demonstrated beneficial roles of these trophic molecules, here, we introduce mesenchymal stem cell-derived exosomes as potent trophic organelles to enhance sperm quality.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/881
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