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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۲-۵۲
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عنوان انگلیسی Ps-75: Preconditioning of Mesenchymal Stem Cells Increases Survival of The Cells: An In Vitro Study
چکیده انگلیسی مقاله Objective: A growing number of experimental studies highlight the potential of stem cell transplantation as a novel therapeutic approach for diseases. This massive loss of stem cells post-engraftment is an impediment that lessens the effectiveness of cell transplantation therapy. Present study tried to increase the survival of the stem cells with H2O2. Materials and Methods: Mesenchymal stem cells were isolated from rat bone marrow. MSCs from passage four were cultured in DMEM medium containing different concentration of H2O2 and 48 hours and then were recovered with fresh medium. Finally treated cells once were exposed with lethal dose of H2O2 (300μM and 500 μM) .The viability of the cells evaluated with trypan blue and proliferation assay with MTT assay. Results: Preconditioning with 5μm H2O2 significantly increased the resistance of these cells against lethal conditions induced by H2O2 (p< 0.05). MTT assay showed higher proliferation in groups with 5 and 10 μM H2O2 after 24 hours. Conclusion: Preconditioning of MSCs with oxidative stresses enhances their survival, therefore it could increase the efficacy of MSCs transplantation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/879
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