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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۲-۵۲
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عنوان انگلیسی Ps-79: Development of A New Wound Dressing;Mixture of Chitosan Hydrogel (CH) and Amniotic Membrane Extract (AME)
چکیده انگلیسی مقاله Objective: Early covering of the burned area is crucial and important factor for successful treatment. Therefore, finding an appropriate Materials for dressing and accelerating wound healing is important. Our previous results determined that amniotic membrane extract (AME) contain many factors that can promote limbal stem cell proliferation and corneal epithelialization. In the present study we investigated the effect of mixture of AME and hydrogel based on chitosan on partial burns in Rat model. Materials and Methods: To find the effective dose of AM extract on skin cells, skin fibroblasts and melanocytes were cultured in 12 well-plates for 24, 48 and 72 hours in presence of different concentration of AME (0.1- 2 mg/ml). Proliferation rate was measured by manual cell count. In next step, 30 Wistar male rats were anaesthetized and a 5×6 cm partial burn wound was created by 90oC water in 6 second, and then divided in four groups. Group1: control (no dressing), group 2: chitosan hydrogel (CH) sheet, group 3: CH contains 1mg/ml of AME up to 10th day then followed by CH contains 0.1mg/ml, group 4: CH contains 0.1 mg/ml AME. In Day 10th and 15 th, biopsies were taken from wound region for pathological evaluation and Masson’s Trichrome (MT) staining. Results: Our results determined that o.1 mg/ml of AME increased fibroblast and melanocyte proliferation significantly (p< 0.05), however 2 mg/ml AME Reduced cell proliferation. On the other hand, dressing of wound by hydrogel contains 1mg/ml AME for 10 continues days and then changed it with hydrogel contains 0.1mg/ml significantly decreased the wound area six fold compare control group (p< 0.01). Pathological experiments showed that collagen fibers increased, inflammatory cells and scar formation decreased in rats which treated with hydrogel contains 0.1mg/ml and 1 mg/ml AME. Conclusion: According to these results, for the first time we introduce a suitable biological dressing without any side effect that accelerates wound healing.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/883
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