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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۳-۵۳
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عنوان انگلیسی Ps-80: In Vitro Differentiation of Human Adult Stem Cells into Hepatic Lineage Cells
چکیده انگلیسی مقاله Objective: Liver pathologies affect hundreds of millions of patients worldwide. Millions of patients worldwide suffer from end-stage liver pathologies, whose only curative therapy is liver transplantation. Procedures of liver transplantation are still limited by the donor scarcity, the high costs, and the lifelong immunosuppressive treatments that they all require. Thus, the development of cell therapies for the treatment of end-stage hepatic diseases is currently under investigation all over the world. Adult stem cell (ASC) therapy could solve the problem of degenerative disorders, including liver disease in which organ transplantation is either inappropriate or there is a shortage of organ donors. Objective: Evaluation of the feasibility and efficacy of human bone marrow mesenchymal stem cells (BM- MSCs) to be separated, characterized by the flow cytometer and in vitro differentiated into hepatocyte like cells using different growth factors. Materials and Methods: 10 ml bone marrow was aspirated from 28 patients. MSCs were identified by their morphology; phenotypic markers were assessed by the flow cytometer using CD73, CD44 and CD34. Isolated MSCs then cultured in differentiating media using different growth factors (b-FGF and HGF) without using extra cellular matrix (ECM). The differentiating ability to hepatocytes was assessed by morphological changes, expression of hepatocyte specific genes (Albumin and Alfa fetoprotein) and also by synthesis & secretion of albumin. The detection of gene expression of Alb and AFP was done by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The cells isolated from BM by density gradient centrifugation and plastic adherence takes the fibroblast like morphology, adherent to the plastic surface and expresses CD73 and CD44 but not express hematopoietic marker CD34. MSCs cultured in differentiating media changed into hepatocyte like morphology, express AFP, Albumin genes and also secrete albumin in the supernatant of culture. Combination of HGF and b-FGF for hepatogenic differentiation of BM-MSCs was better than single factor alone. Conclusion: Our study proved the possibility of isolation of BM- MSCs and in vitro induction into cells expressing most important characteristics of functioning hepatocytes. Our thesis implied the hope of possible therapeutic application of such hepatic lineage against liver diseases in the future. Stem cell research is being pursued vigorously in our laboratories in the hope of achieving major medical breakthroughs in the future.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/884
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