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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۳-۵۳
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عنوان انگلیسی Ps-81: Preparation of Testicular Cell Conditioned Medium from Rat Testis
چکیده انگلیسی مقاله Objective: In vivo, growth factors that mediate local cellcell interactions are responsible for the differentiation of primordial germ cells (PGCs) into germ stem cells after the arrival at the genital ridge. in vitro differentiation of PGCs is also dependent on their sequential exposure and response to an array of growth factors. In the presence of multiple growth signals, PGCs restart rapid proliferation in vitro and transform into pluripotent embryonic germ cells (EGCs). Hence, some cases of sterility are due to lack of germ cells, in the future, this problem could be treated using stem cell therapy. Thus, in this study we effort the preparation of testicular cell conditioned medium from rat testis. Materials and Methods: Testes of 1-day-old newborn male rats were removed and placed into tripsin-EDTA solution. Then the harvested cells cultured with DMEM medium supplemented with 10% FCS, 1% nonessential amino acids, and 1% penicillin/streptomycin. After proliferation of germ cells, testiculat conditioned medium was collected every 3 days starting 10 days after initiation. Results: An obvious germ cell proliferation was evident in TCCs 8-10 days from initiation. The round cells were clustered together and easily dissociated from other cells when dishes were shaken. Germ cells differed in size and appeared as single cells or attached to each other, forming pairs or rows. Conclusion: Testis, particularly of newborns, contains germ cells that release growth factors during in vivo culture. In this regard, we try the in vivo differentiation of mesenchymal stem cells of rats into PGCs and precursors of sperm by TCC medium induction. Their results will be announced later.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/885
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