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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۴-۵۴
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عنوان انگلیسی Ps-82: Evaluating the Expression of miR-491 As A Potential Stemness Marker in Lung Tumor and Non-Tumor Tissue
چکیده انگلیسی مقاله Objective: MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate expression of target mRNAs. The biological processes modulated by miRNAs include cell differentiation, proliferation and apoptosis. It is approved that abnormal expression of miRNAs could cause various diseases, including cancer. P53 is a master negative regulator of Nanog expression which is a key to maintenance of pluripotency in stem cells, thus loss of p53 promotes stemness and selfrenewal. miR-491 is known to negatively regulate TP53 therefore its aberrant expression could negatively affect TP53 regulatory pathway. Considering the potential role of miR-491to miss-regulate p53 and based on cancer stem cell (CSCs) hypothesis, deregulation of miR-491 might ignite the tumorigenic process in adult stem cell and transform them into cancer stem cell state. The aim of our study is to find a potential link between the expression of miR-491 and tumor formation as a result of CSCs activation in lung tissue. Materials and Methods: In this study, RNA was extracted from lung tumor and non-tumor tissues. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays were performed with specific stem-loop primer and EVA green master mix. The ability of primer to amplify specific microRNA was evaluated. Results: According to our data, stem-loop primer could amplify miR-491-5p specifically. RT-PCR results showed that expression level of miR-491-5p could be evaluated in lung tumor and non-tumor tissue. In addition, expression level of miR-491-5p might be significantly different in lung tumor and non-tumor tissue. Conclusion: Our data suggested a potential role of miR- 491-5p in tumorgenecity. According our data and previous data we can say that miR-491 might have a role in stemness of stem cell and cancer stem cell.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/886
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