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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۴-۵۴
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عنوان انگلیسی Ps-83: Evaluating The Expression Alteration of miR-296 As A Stem Cell Specific Marker in Lung Tumor
چکیده انگلیسی مقاله Objective: MicroRNAs (miRNAs) are a group of noncoding regulatory RNA involved in diverse biological processes, including development, differentiation, proliferation, and apoptosis. miRNA genes are commonly found in cancer-associated genomic regions and their expression pattern alterations are reported in several cancers, including lung cancer. miR-296 is a stem cell specific microRNA which is located in 20q13.32 genomic locus. By independent experiment on genomic analysis of cancer cell lines, it is shown that this chromosome region is amplified in lung cancer. Furthermore, miR-296 is up regulated during human cell immortalization and in embryonic stem cells. According to the cancer stem cells (CSCs) hypothesis, aberrant expression of such a gene might contribute to tumorigenicity. Herein, our aim of study is to evaluate the expression alteration of miR-296 as a cancer stem cell specific marker in lung tumor tissues. Materials and Methods: To investigate miR-296 expression pattern in a matched case-control way, formalin- free paraffin-embedded (FFPE) samples of lung tumor along with matched non-tumor margin were obtained. Paraffin was removed using the xylene-ethanol method then prepared tissues were treated by proteinase K to eliminate inhibitory proteins. Total RNA was isolated using TRIzol reagent. Specific cDNA synthesized by means of stem-loop primer and miRNA expression assessed by real-time PCR. Results: Our data revealed that designed stem-loop primer was highly specific and could succesfully assess the expression alteration pattern of miR-296 in lung tumor versus non-tumor samples. Conclusion: According to our primary set of data, the expression of miR-296 in lung tumor could claim the presence of CSCs in tumor cell population. However, introducing miR-296 alteration expression as a discrimination factor of tumor state is under study and needs further investigation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/887
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