این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۶-۵۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-86: Biomimetic Scaffold to Enhance Chondrogenic Differentiation of Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: In this study, the differentiation behavior of Mesenchymal Stem Cells (MSCs) onto the surfaces of blended semi-interpenetrating polymer network(semi- IPN) scaffolds consist of Polycaprolactone (PCL), Polyvinyl alcohol (PVA) and Gelatin (GEL) in order to mimic natural cartilage extracellular matrix were investigated. Materials and Methods: PCL-PVA-GEL blended semi- IPN scaffolds were prepared by mixing aqueous and nonaqueous polymer solutions followed by homogenizing, and freeze-drying. Then, the scaffolds were characterized by SEM, compressive mechanical test, and biological assays of MSCs culture, MTT, DMMB, and AO/PI. Results: Cartilage lesions are usually irreparable and natural physiological response is not effective on their treatment. Therefore, tissue engineering using polymeric scaffolds in order to create the tissue with similar properties to the cartilage is an interesting and promising procedure. In this study, the results showed using PCL-PVA-GEL composition with aforementioned procedure could lead to an open interconnected porous structure with similar modulus (1.27 ± 0.04 MPa) to the natural human cartilage tissue. The surface of scaffolds showed an excellent efficiency in the adhesion and proliferation of MSCs. The increase of cell proliferation and secretion ECM proteins was achieved through affinity of cells possibility toward the GEL containing matrix. A significant increase in proteoglycan content from 5.4 ± 1.13 μg/ml to 9.81 ± 1.74 μg/ml was observed after 21 days. The results of AO/PI test revealed more than 90% of cells were alive inside the scaffolds. Conclusion: The study revealed that the aforementioned scaffold as a blend of natural and synthetic polymers may be used as a promising substrate in tissue engineering for cartilage repair using MSCs transplantation.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/890
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات