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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۶-۵۶
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عنوان انگلیسی Ps-87: Effects of Hydrostatic Pressure on Chondrocyte Biosynthesis in Polycaprolactone-Polyvinyl Alcohol-Gelatin Scaffolds
چکیده انگلیسی مقاله Objective: This study was undertaken to determine the effects of hydrostatic pressure on the biochemical properties and gene expression of mesenchymal stem cells (MSCs) onto PCL (polycaprolactone) -PVA (polyvinyl alcohol)-GEL (Gelatin) semi IPN scaffolds in cartilage tissue engineering. Materials and Methods: The MSc cells were seeded onto PCL-PVA-GEL semi IPN scaffolds in two groups (A and B) for 7 and 21 days, respectively, and then loaded with hydrostatic pressure (5 MPa, 0.5 Hz) for 2 hours per day for the period of 7 days and compared with two non-loaded groups (C and D) as control. DMMB and Real-time PCR analysis for assaying cartilage-specific ECM gene markers were carried out. Results: Mechanical stimuli such as hydrostatic pressure, which plays a considerable role in the mechanoregulation of cartilage, can be sensed by chondrocytes in articular cartilage. According to the results, although no significant differences for GAG amount between the loaded and non-loaded constructs was observed after 14 days, significant and considerable increases in the expression amount of type II collagen mRNA levels in group A (from 2.43×10-4 ±5.32×10-5 to 2.09×10-3 ±1.07×10- 4 time), and in group B (from 3.04×10-4 ±4.31×10-5 to 2.08×10-3 ±1.59×10-4 time) in comparison with nonloaded groups (C and D) were observed, respectively. On the other hand, neither significant increase in aggrecan nor in sox9 expression after 14 days was found. Conclusion: Results showed the beneficial role of hydrostatic pressure on the increase of type II collagen mRNA levels in articular cartilage tissue engineering.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/891
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