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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۸-۵۸
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عنوان انگلیسی Ps-91: Evaluation of Cytokines Produced by Mitogen Stimulated Bovine Peripheral Blood Mononuclear Cells (bPBMCs)
چکیده انگلیسی مقاله Objective: This study was conducted to evaluate cytokine production by bovine peripheral blood mononuclear cells stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (ConA). The production of IL-2, IL-4, IL-5, IL-6, IL-10, IL-17 and gamma interferon was measured in the supernatant of stimulated cells. The 2×105 cells were cultured in the presence of various concentration of mitogens and cytokine production was assessed using commercially available sandwich ELISA kits. Materials and Methods: According to the results presented in Table 1 and figure -3-1, IL-2 IL-4, IL-5, IL- 10, IL-17 and IFN-γ production in response to PWM mitogens was highest and Con A lowest amount and the median values of three mitogens were in the following order: PWM > PHA > ConA > cell control. In the case of IL-6, the production of this cytokine was the same amount for PWM and Con A and lower amount for PHA stimulation. Results: The results of this study not only showed a normal range for the production of these cytokines from PBMCs that were affected by mitogens, but it demonstrated that the bovine immune system at 2.5-3 months is postnatally mature enough to mount an effective immune response to mitogens as well as specific antigens Conclusion: The results ,demonstrated that the bovine immune system at 2.5-3 months is postnatally mature enough to mount an effective immune response to mitogens as well as specific antigens.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/895
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