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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۵۸-۵۸
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عنوان انگلیسی Ps-92: The Influence of Cerebrospinal Fluid on Epidermal Neural Crest Stem Cells May Pave the Path for Cell-Based Therapy
چکیده انگلیسی مقاله Objective: A wide range of therapeutic approaches have been evaluated for treatment of central nervous system (CNS) injuries and neurodegenerative disease and epidermal neural crest stem cell (EPI-NCSC) as an adultresident multipotent stem cell in the bulge of hair follicle has shown a number of advantages which makes it an appropriate cell type for transplantation. A prominent factor in cell based therapy is the practicalities of different routes of administration. Cerebrospinal fluid owing to its adaptive library of secreted growth factors can provide a trophic environment for transplanted cells. Materials and Methods: In this study, the highly pure population of EPI-NCSC was obtained by virtue of its migratory ability from bulge of hair follicles. Cells were characterized by RT-PCR and immunocytochemistry. Subsequently isolated stem cells were cultured in CSF collected from cisterna magna of adult rat. Isolated cells were treated with CSF for 72 hours and then the expression of pertinent markers was assessed at the gene and protein level by real-time PCR and immunocytochemistry. Also Colorimetric immunoassay quantified the rate of proliferation. Results: Our results revealed that isolated EPI-NCSC could survive in the CSF and they proceeded expression of Nestin, β - Tubulin ІІІ (early neuronal marker) and GFAP (glia marker) in this enviroment. Also colorimetric BrdU ELISA kit indicated CSF decreased significantly the proliferation rate of EPI-NCSC compared to expansion culture medium (p< 0.01). Conclusion: Alltogether, CSF as a cocktail of growth factors helps EPI-NCSC to acquire some desirable traits and due to its circulatory system that is in close contact with different parts of CNS, can be a practical route of administration for delivery of injected stem cells into injured spinal cord.Moreover, low proliferation rate of these stem cells in CSF is an attractive feature in cellbased therapy.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/896
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