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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۱-۶۱
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عنوان انگلیسی Ps-97: Study on Lactobacillus of European Cat Fish (Silurus Glanis) Intestine in Siahdarvishan River of Iran by PCR Method
چکیده انگلیسی مقاله Objective: Lactic acid bacteria (LAB) are constituted of a heterogeneous group of Gram-positive bacteria with a strictly fermentative metabolism from which lactic acid is a key metabolite. Materials and Methods: During present research about 50 European cat fish (Silurus glanis) were hunted from siahdarvishan river (Bandar Anzali, Iran) randomly. The samples were transported to the Microbiology laboratory under sterile conditions and in the vicinity of ice. After dissection according to sterile Method; 1 gram of stool was sampled from the anterior part of intestine and cultured in MRS Agar plates. Phonotypical and biochemical of Bacteria was identified for molecular characterization distinction of lactobacillus species. 16SrDNA gene was amplified and after electrophoresing cutting pattern of 16s rRNA, compared by Gen Doc program with cutting pattern of 16s rRNA gene sequences of species which recorded in the Gene Bank (NCBI). Results: In biochemical tests, 15 samples were positive for Lactobacillus, 7 cases of them were lactobacillus plantarum and 3 cases were lactobacillus casei. Cutting patterns of 5 other samples were different from L. Casei, L. plantarum, and even from cutting patterns of 16s rRNA genes of lactobacillus that have been recorded in Bioinformatics. Conclusion: Results indicated 30 percent of European Cat Fish samples were positive for Lactobacillus and the most cases were lactobacillus plantarum.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/901
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