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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۳-۶۳
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عنوان انگلیسی Ps-100: Evaluation of Cardiac Differentiation Potential of Menstrual Blood versus Bone Marrow-Derived Stem Cells
چکیده انگلیسی مقاله Objective: Recently, identification of menstrual bloodderived stem cells (MenSCs) as a unique source of stem cell with some characteristics as well as ease of access, high proliferative ability and renewability has created enormous promise for cell therapy. In this study, differentiation ability of MenSCs into cardiomyocytes has been compared with that of bone marrow-derived stem cells (BMSCs). Materials and Methods: After characterization of Men- SCs compared with BMSCs using flow cytometry, their differentiation into cardiomyocyte was investigated in the presence of 5-azacytidine and basic-fibroblast growth factor. The expression of the putative myogenic cells at mRNA and protein levels was determined by immunofluorescent staining and real-time quantitative PCR. Results: Based on flow cytometric analysis, the isolated MenSCs typically expressed mesenchymal stem cell markers like CD105, CD73, CD44 and CD166 and lack hematopoietic stem cell markers such as CD34, CD45 and CD133 in a similar manner with BMSCs. However, in contrast to BMSCs, MenSCs exhibited marked expression of OCT4 related to embryonic markers. The both differentiated MenSCs and BMSCs expressed cardiomyocyte markers at mRNA/protein level. While, the expression level of some cardiomyocyte markers such as Connexin- 43 and troponin T2 (TNNT2) protein were higher in differentiated MenSCs compared to BMSCs, there was no significant difference between mRNA levels of Connexin- 43, Alpha actinin ,Tropomyosin1 and TNNT2 of differentiated MenSCs and differentiated BMSCs. Conclusion: Based on our data, MenSCs are a unique cell population with differentiation ability into cardiomyocyte- like cells. However, the pattern of cardiac markers at mRNA and protein level in differentiated MenSCs is relatively different with that of derived BMSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/904
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