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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۴-۶۴
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عنوان انگلیسی Ps-103: Introduction of Scleroderma-Specific Induced Pluripotent Stem Cells in A Dish: An Unlimited and Available Source for Evaluation of Developmental Defects in Regenerative Medicine
چکیده انگلیسی مقاله Objective: Scleroderma -one of the important autoimmune diseases- leads to death of endothelial cells as one of the early events of this disease. The lack of repair after the loss of endothelial cells is observed in these patients and although it is a worldwide disease, its cause has remained unknown. Because of physiological differences between human and other species, a proper scleroderma animal model which represent all aspects of the disease has not been generated yet. So, making a patient-based system to mimic a developmental defect and evaluating its probable repair mechanism is considered as a necessity. In this study it was tried to generate patients’ specific induced pluripotent stem (iPS) cells and characterize them. Materials and Methods: Patient’s fibroblast cells -as an available and unlimited source of disease study in vitrowere isolated, cultured and transduction with retroviral vector containing Oct 3/4, Sox2, Klf4 and c-Myc. For characterization of generated iPS cells, their expression of endogenous pluripotent and silencing of retroviral genes were evaluated by RT-PCR technique and the existence of pluripotency proteins were assessed by immunostaining. Spontaneous differentiation into endothelial cells was done by embroid body formation and the expression of specific endothelial cell markers were examined by flowcytometery technique. Results: RT-PCR analysis and immunostaining study of generated cells compared to human embryonic stem cells (royan H5) results showed that reprogrammed cells express specific markers similar to embryonic stem cells. EB formation results And also, spontaneous differentiation into endothelial cells using EB formation showed that our scleroderma derived pluripotent cells have differentiation potential like embryonic stem cells. Conclusion: Our study showed that scleroderma specific iPS cells can be indicated as an unlimited and available source for assessing molecular defects in vitro and finding regenerative methods to repair endothelial cells in scleroderma patients.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/907
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