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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۵-۶۵
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عنوان انگلیسی Ps-106: Effect of Adipose Derived Stem Cells (ASCs) on EMT Specific Markers after Transfection of MDA-MB-468 Cell Line by An Anti-Angiogenic Chemokine
چکیده انگلیسی مقاله Objective: Epithelial-mesenchymal transition (EMT) is an important mechanism in tumor progression and metastasis. In addition to the uncontrolled epithelial proliferation and angiogenesis, the role of this mechanism in solid tumors such as breast cancer is also important for cancer progression and metastasis. The role of cytokines such as TGF-β and SDF-1 in triggering the EMT mechanism is previously demonstrated. In contrast to the SDF- 1 with angiogenic roles, the chemokine IP-10 has been documented with anti-angiogenic activities but there is no research which shows the effect of IP-10 on EMT. Thus, in this study we investigated the effect of IP-10 overexpression on EMT mechanism in tumor cells. Besides, because of the key role of tumor microenvironment on tumor progression and the importance of ASCs, we also investigated the effect of these cells on the EMT mechanism. Materials and Methods: ASCs were isolated from 6 breast cancer patients and 6 normal individuals and then cultured. MDA-MB-468 breast cancer cell line was transfected by IP-10 plasmid through electroporation. Transfected cell line was co-cultured with ASCs using transwell system. After 4 days, proteins were extracted and the expressions of MMP9 and MMP13 were defined by western blotting. Results: Results of western blot for MMP9 and MMP13 protein expressions showed that the expression of both proteins were downregulated in IP-10 transfected MDAMB- 468 cells compared to the untransfected cells. Presence of ASCs causes the upregulation of MMP9 in MDA-MB468 cell line. Conclusion: Based on the results of this study, IP-10 may downregulate the EMT markers in MDA-MB-468 cancer cell line. No different was found between the ASCs from different sources but the presence of ASCs in tumor microenvironment can lead to the up-regulation of EMT markers. Accordingly, IP-10 may use as an antitumor therapeutic agent to suppress tumor progression and metastasis.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/910
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