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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۸-۶۸
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عنوان انگلیسی Ps-110: Isolation and Culture of Neonatal Rat
چکیده انگلیسی مقاله Objective: Oligodendrocyte is a type of neuroglia cells that plays an important role in transmission of nerve signaling over the axon to the central nervous system in vertebrates. This action is employed by production and wrapping myelin sheath around the axon. Demyelination which is a result of myelin sheath's destruction following elimination or reduction supportive role of oligodendrocyte has been reported in diseases such as multiple sclerosis or progressive multi focal leukoencephalopathy (PML). Nowadays, with improvement of knowledge in use of stem cells for the treatment of different diseases, many research projects have been designed in order to the isolate, culture and differentiate oligodendrocyte precursor cells (OPCs) in vitro. The purpose of this study is isolating of OPCs and transferring them into the brain and spinal cord of newborn rat with a genetic defect in myelin production. We are expecting the new OPCs migrate and differentiate to oligodendrocyte cells in the rats. Materials and Methods: To set up the project, the brain cortex tissue of newborn rat would be used. After standard procedures, homogenous cell population of OPCs would prepare in a defined culture medium supplemented with factors such as bFGF and pDGF. To differentiate OPCs from the other cell population in culture medium, Immunohistochmeical assays and some specific OPCs markers would also be investigated. Following in vitro isolation and differentiation of the OPCs we would be able to study various biological and immunological mechanisms diseases related to the OPCs. Results: If OPCs can be cultured and maintain in vitro, then we would be able to study, different biochemical, physiological and immunological mechanisms affecting these type of neural cells. On the other hand, the ability of oligodendrocytes in myelin production in vivo would also be investigated. Conclusion: The results would show if we would be able to use OPCs for myelin substituation in patients with myelin sheath's destruction.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/914
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