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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۶۹-۶۹
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عنوان انگلیسی Ps-114: Direct Conversion of Human Fibroblasts into Functional Neural Cells Using Reprogramming Strategies
چکیده انگلیسی مقاله Objective: Cell fate conversion is not a dream anymore. Not only it is biologically feasible today, but also it is adopted as a new tool in cell biology research within a short time. For several years it was believed that the fate of each individual cell which is established during development is irreversible and irrecoverable due to illness and injury. Successful reprogramming of somatic cells to a pluripotency state using only four transcription factors raises the question whether it is possible to convert a fully differentiated cell type into another directly in vitro, without any additional step like going back to pluripotent state. Our purpose in this project is develop a directed transdifferentiation protocol, for generating multipotent, neural lineage restricted, and nontumorigenic iNSCs from human fibroblasts by direct reprogramming strategy.Materials and Methods: At first step human foreskin fibroblasts were infected with Retrovirus containing the Yamanaka factors for 24 hrours, then they were cultured in a chemically defined medium containing Dorsomorphin, CHIR99021 and SB431542. Results: Induced neural progenitor (iNP) colonies appeared as early as day 13. Many of colonies which stained positive for PAX6 and NESTIN and can be differentiate into mature and functional neurons that express TUJ1 and MAP2. Conclusion: The ability to generate defined, induced neurons from human fibroblasts under a defined condition with predictable fate choices will facilitate disease modeling and therapeutic development.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/918
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