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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۳-۷۳
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عنوان انگلیسی Ps-122: CDH1, CD44 and CD49b As Markers for Identification and Isolation of Prostate Cancer Stem-Like Cells
چکیده انگلیسی مقاله Objective: Prostate cancer that has progressed to metastatic disease remains largely untreatable. Most solid tumors originate from rare tumor cells which are responsible for initiation, progression and tumor metastasis are named cancer stem (like) cells and they may be attractive targets for cancer therapy. Mounting evidence has demonstrated that aggressive and metastatic cells share molecular characteristics with cancer stem like cells such as CD44, a2ß1 integrin and CD133. In this study, we aimed to find whether there is any correlation between stemcell- related functions and expression level of stemness and metastasis genes. Materials and Methods: Prostate cancer stem-like cells were isolated from PC3 cell line by spheroid formation as well as expression detection of some surface markers (CD44, CD49b) using BD FACS Aria II. To characterize the sorted cells, doubling time, colony formation potential and sphere formation were done on each group and also the parental line. Quantitative RT-PCR was used to assess the expression level of stemness genes (OCT4, SOX2, NANOG, c-MYC, KLF4) and metastasis genes (CDH1 and CDH2) between tested groups (CD44+/ CD49bhi, CD44-/CD49blow and spheres), and parental cells. Results: PC3 line, CD44+/CD49bhi and CD44-/CD- 49blow formed three different phenotypic of colonies are named holo, mero and para clones. The number of holo and mero clones were higher in CD44+/CD49bhi cells compared to CD44-/CD49blow group (30.2 vs. .27% and 21.95 vs. 2.81%); whereas the number of paraclones were higher in CD44- cells (3.8 vs. 2.3%). The proliferation rate and spheroid formation efficiency were higher in PC3 cells (32 hours/ 0.46%) and CD44+/ CD49bhi (29.65 hours/ 0.55%) compared to CD44-/CD- 49blow group (49.18 hours/ 0.04%), respectively. There was a significant increase in the expression of c-MYC in CD44+/CD49bhi and KLF4 in CD44-/CD49blow groups. The expression of CDH1 was significantly increased in spheroid and CD44+/CD49bhi cells compared to the CD44-/CD49blow ones. Conclusion: Our data revealed that the subpopulation of CD44+/CD49bhi cells had cancer stem-like cell properties with higher expression of CDH1, therefore we suggest that the E-cadherin, CD44 and CD49b can be considered as suitable markers for identification and isolation of prostate cancer stem-like cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/926
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