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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۴-۷۴
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عنوان انگلیسی Ps-124: Transdifferentiation of Human Adipose Tissue-Derived Stem Cells to Dopaminergic Neurons
چکیده انگلیسی مقاله Objective: Adipose tissue-derived stem cells (ADSCs) have the capability of differentiation to mesodermal, endodermal and ectodermal lineages. Several studies have demonstrated the differentiation of ADSCs to neuronal and glial phenotypes. The aim of present study was to investigate dopaminergic differentiation of ADSCs in vitro. Materials and Methods: Human ADSCs were isolated from the adipose tissues collected from patients who underwent abdominoplasty. ADSCs were characterized by flow cytometric analysis. For dopaminergic differentiation, we used a cocktail of sonic hedgehog (SHH) and fibroblast growth factors under a low serum condition. As control, ADSCs were cultured in low serum medium without induction cocktail. After 12 days of differentiation, study of gene expression was performed by RT-PCR and quantitative real-time RT-PCR (qPCR). Immunocytochemistry was used to assess the expression of dopaminergic marker, tyrosine hydroxylase (TH), and mature neuronal marker, neurofilament light polypeptide (NEFL). Results: Flow cytometric analysis confirmed the mesenchymal nature of the ADSCs. RT-PCR study showed the expression of dopaminergic neuron-specific-genes, including TH, EN1, NURR1 and PITX3 mRNAs, in the treatment group. The control group only showed the expression of EN1 and NURR1 mRNAs. QPCR analysis demonstrated upregulation of TH and NURR1 mRNAs in the treatment group compared to control group. Immunostaining revealed the expression of TH and NEFL proteins in the differentiated ADSCs. Conclusion: Adipose tissue is an abundant source of adult stem cells with the potential for differentiation to dopaminergic neurons. ADSCs might be a valuable source for the future cell replacement therapy especially in regards to disorders involving loss of dopaminergic neurons.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/928
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