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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۵-۷۵
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عنوان انگلیسی Ps-126: Establishment and Biological Evaluation of The Goat Fetal Fibroblast Cell Line
چکیده انگلیسی مقاله Objective: Fibroblast cell lines have different application in biological research including nuclear transfer in cloning, feeder layer of embryonic stem cells, wound healing research and tissue engineering. The objective of the present study was to establishment and biological evaluation of goat fetal fibroblast before and after cryopreservation. Materials and Methods: Goats fetus (age about 51, 53 and 55d) were collected from Shiraz Slaughterhouse. Their skin was cut into small pieces (1 mm3) and cultured in DMEM and FBS. When reaching 80-90% confluence, the cells were passaged. Cells of the 8th passage were cultured in 24-well plates (1.5×105 cells/well) for 9 days and three wells per day were counted. The average cell counts at each time point were then plotted against time and the duplication time (PDT) was determined. Then, 42 vials of cells (4×106 cells/ml) were cryopreserved for 1 month and cultured after thawing. Cell viability and PDT of the cells were evaluated after thawing. Results: The goat fetal fibroblasts after passage had a latent phase of about 48 hours. After an exponential phase, cells entered the plateau phase on day 5. Before freezing, PDTs were 59.7 hours on day 51, 90.6 hours on day 53, and 53.1 hours in day 55 of pregnancy. After freezing, PDTs were 54.6 hours on day 51, 52.1 hours on day 53, and 65.5 hours on day 55 of pregnancy. The freezing had a little effect on the viability of the cells. Conclusion: The goat fetal fibroblast line was established using the adherent culture method and cryopreserved. After freezing, growth and viability indices of the cells were favorable.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/929
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