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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۶-۷۶
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عنوان انگلیسی Ps-130: RNA Microarray Analysis Showed Significantly Changed Gene Expression after In Vitro Human Cardiac Stem Cells and Satellite Cells Passages
چکیده انگلیسی مقاله Objective: Increasing rate of morbidity and mortality by heart diseases all over the world attracted scientists’ attention to this important medical case. In recent years regenerative medicine has been introduced as an alternative tool for heart repair; and cardiac stem cells (CSCs), precursor cells resident in heart tissue have been investigated as novel sources of heart cell therapy. Such patient derived stem cells need to be isolated from small heart biopsies and expanded in culture to reach the quantity which is required for cell therapy. Although various aspects of adult stem cell transplantation have been well studied, the effects of extensive sub-culturing on the characteristics and phenotype of these cells have remained elusive. Materials and Methods: Human CSCs and skeletal muscle stem cells (satellite cells) were isolated from heart and intercostals biopsies of patients with congenital diseases during routine heart surgeries with informed consents. Isolated cells were passaged until their morphologies began to change and they were sampled at 4 passages -passage 6, 9, 12 and 15. Cell surface markers’ changes were analyzed using flowcytometery system and whole genome transcriptom alteration was assayed using Illumina chip microarray analysis. Results: Doubling time measurement results showed that subsequent passages caused an increase in essential time for cell number duplication and flowcytometry analysis indicated the different cell surface markers at different passages. Whole genome analysis showed gene expression alteration by increasing the passage number (ANOVA p value≤0.05, fold change≥2). The most important gene ontology which significantly altered during passages was cell cycle. Conclusion: Our study showed that subsequent passages cause significant changes in both phenotypic and genome expression characteristics of CSCs and satellite cells. As both CSCs and satellite cells are considered as stem cell therapy sources, it’s important to decrease any cell modification in culture.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/932
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