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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۸-۷۸
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عنوان انگلیسی Ps-134: WNT3A Signaling Pathway Has Significance to Maintain Undifferentiated State of Buffalo Embryonic Stem Cells
چکیده انگلیسی مقاله Objective: The present study was aimed to study the effects of WNT3A signaling pathway on buffalo embryonic stem (ES) cells. WNT proteins play roles in the regulation of gene expression, cell proliferation, and differentiation and in the maintenance of cell polarity. Materials and Methods: Data were analyzed by ANOVA, and statistical significance was accepted at p< 0.05. Results: The increase in mean colony area between days 1 and 6 was significantly lower (p< 0.05) with 400 ng/mL than that with 0 (control), 100 or 200 ng/mL WNT3A. The increase in mean colony area was significantly higher (p< 0.05) in the presence of WNT3A+FGF-2+LIF than in the presence of FGF-2+LIF which, in turn, was higher (p< 0.05) than when only WNT3A was present or that in the controls. Real-time qPCR analysis showed that the relative mRNA abundance of β-CATENIN was significantly increased (p< 0.05) and that of SOX2, OCT3/4 and C-MYC was not affected by WNT3A supplementation at all the concentrations examined. Both WNT3A and FGF- 2+LIF, either alone or in combination, significantly increased (p< 0.05) the expression level of β-CATENIN and NANOG compared to that in the control. Conclusion: WNT3A works together with exogenous FGF-2 and LIF resulting in proliferation of undifferentiated buffalo ES cells, however, in absence of the exogenous factors it leads to no significant affect on proliferation of buffalo ES cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/936
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