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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۸-۷۸
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عنوان انگلیسی Ps-135: A Modified Method for Cerebrospinal Fluid Collection in Anesthetized Rat and Evaluation of The Efficacy
چکیده انگلیسی مقاله Objective: The aim of present study was to propose a simplified technique for efficient collection of CSF from cisterna magna of the adult rats. Materials and Methods: Twenty three male Wistar rats with the body weight range of 200-300 g were enrolled to the study. Rats were anesthetized by ketamine(50 mg/kg) and xylasine 2% intraperitoneally. Specially constructed ear bars were placed in the external auditory meatus and the rats were placed in a stereotaxic head holder. The head was flexed downward at approximately 90◦, a depressible surface with the appearance of a rhomb between occipital protuberances and the spine of the atlas becomes palpable. A midline scalp incision was made and the cervico-spinal muscle was reflected and the atlanto-ocipital membrane exposed. Using a special stereotaxic guide to hold the syringe, the point was carefully advanced under direct vision. The atlanto-occipital membrane punctured then by a gentle aspiration, the non-contaminated CSF was drawn into the syringe. Results: Twenty three rats were obtained for CSF collection. The successful index was the collection of clear and colorless CSF. In 13 rats, we could successfully aspirate the CSF. The total volume was 1200 micro liters (varies from 80 to150 μl per animal). The success rate was 76.4%. Failure etiologies were mainly related to dry aspiration, blood contaminated CSF, obstruction of the needle. Conclusion: This simple technique represented efficient method for experiments in which non contaminated CSF is required. The technique can be easily done by anyone who is familiar with stereotaxic surgery, and the required Materialss are cheap and easy to obtain.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/937
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