این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۷۹-۷۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-137: Differential Expression Level of Nestin in Mesenchymal Stem Cells Derived from Human Chorion and Bone Marrow
چکیده انگلیسی مقاله Objective: Nestin is an intermediate filament protein that has been extensively used as a marker for central nervous system (CNS) progenitor cells in different studies. On the other hand, Mesenchymal stem cells (MSCs) are originally known to possess capacities to differentiate into mesenchymal cell lineages including bone, cartilage and adipose cells. Furthermore according to previous studies, Nestin positive MSCs tended to differentiate into neuronal cell lineages in vitro. In the search for other suitable source for MSCs, in this study we tried to isolate stem cells from human chorion and compared them to those of bone marrow (BM) in terms of differentiation capacities. Moreover we compared the two cells with respect to the expression level of Nestin which is an indicator of cell potential in differentiating into neural cell lineages. Materials and Methods: Chorionic plate was isolated from patients undergoing cesarean and subjected to the enzymatic digestion using collagenase IV. Released cells were then plated and cultured. Bone marrow was obtained from patients volunteered for cell therapy at Royan Institute. Passaged-3 cells were compared in terms of surface epitopes and multilineage differentiation potential. The expression level of Nestin in BM-MSCs and chorion stem cells (CSCs) were evaluated quantitatively by RT-PCR technique. Results: Immunophenotyping of BM-MSCs and CSCs demonstrated that the majority of both cells were positive for mesenchymal markers (CD105, CD44, CD90, and CD73). Hematopoietic cell lineage markers (CD34 and CD45) were expressed on small percentages of the cells. Both cells succeeded to differentiate into mesenchymal lineages. According to our findings, CSCs showed significantly higher expression level of Nestin in comparison to BM-MSCs (p< 0.05). Conclusion: Consequently, since CDSCs have expressed Nestin in high levels, it would be regarded as a source of stem cells appropriate for cellular therapy in neurological disorders.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/939
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات