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Cell Journal، جلد ۱۵، شماره Suppl ۱، صفحات ۸۰-۸۰
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عنوان انگلیسی Ps-139: Pioglitazone Enhances Dissociated Human Embryonic Stem Cells Self-Renewal Along with FGF2 Through Increasing Nanog Expression
چکیده انگلیسی مقاله Background: Self-renewal in hESCs , required for maintenance of embryonic stem cells (ESCs) in an undifferentiated pluripotent state. SOX2, OCT4 and NANOG are transcription factors which require for the selfrenewal machinery of hESCs. As proved earlier, FGF2 induces nanog expression and has an inhibitory effect on neural induction specifically through decreasing effect on PAX6 expression in hESCs. In the recent study, we demonstrated that peroxisome proliferator-activated receptor gamma agonists have a pivotal role on self- renewal of mESCs. In the present study, we focused on effects of Pioglitazone implementation as a PPAR gamma agonist, on self-renewal factors and neural precursor markers along with FGF2 signaling pathway in dissociated hESCs. Materials and Methods: Human pluripotent cells RH5 cell line were grown in serum-free N2B27-based media. these cells were treated with pioglitazone in presence or absence of bfgf during 4 days and expression level of pluripotent markers was assessed by Real time PCR .localization and intensity of these markers were shown with immune staining. Results: Implementation bFGF with Pioglitazone increased Nanog expression, whereas did not any significantly effect on OCT4 or SOX2 expression as compared with bFGF supplementation. Moreover, pioglitazone decreased Pax6 and Sox1 as neural precursor cell markers. Conclusion: We demonstrated pioglitazone could positively affect on NANOG, downstream of FGF2 and decreased PAX6 and SOX1 neural precursor genes, which lead to improvement of hESCs self –renewal.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/941
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